Regulation of nuclear lamin polymerization by importin alpha.
(2008)
283:8462-8468.
Abstract
Nuclear lamins are integral components of the nuclear envelope and are important for the regulation of many aspects of nuclear function, including gene transcription and DNA replication. During interphase, the lamins form an intranuclear intermediate filament network that must be disassembled and reassembled when cells divide. Little is known about factors regulating this assembly/disassembly cycle. Using in vitro nuclear assembly and lamin assembly assays, we have identified a role for the nuclear transport factor importin a in the regulation of lamin assembly. Exogenous importin a inhibited nuclear lamin assembly in Xenopus interphase egg nuclear assembly assays. Fractionation of the egg extract used for nuclear assembly identified a high molecular weight complex containing the major egg lamin , XLB3, importin a and importin ss. This complex could be dissociated by RanGTP or a competing NLS indicating that lamin assembly is Ran and importin a-dependent in the egg extract. We show that the addition of importin a to purified lamin B3 prevents the assembly of lamins in solution. Lamin assembly assays show that importin a prevents the self-association of lamins required to assemble lamin filaments into the typical paracrystals formed in vitro These results suggest a role for importin a in regulating lamin assembly and possibly modulating the interactions of lamins with lamin-binding proteins.
Nuclear lamins are integral components of the nuclear envelope and are important for the regulation of many aspects of nuclear function, including gene transcription and DNA replication. During interphase, the lamins form an intranuclear intermediate filament network that must be disassembled and reassembled when cells divide. Little is known about factors regulating this assembly/disassembly cycle. Using in vitro nuclear assembly and lamin assembly assays, we have identified a role for the nuclear transport factor importin a in the regulation of lamin assembly. Exogenous importin a inhibited nuclear lamin assembly in Xenopus interphase egg nuclear assembly assays. Fractionation of the egg extract used for nuclear assembly identified a high molecular weight complex containing the major egg lamin , XLB3, importin a and importin ss. This complex could be dissociated by RanGTP or a competing NLS indicating that lamin assembly is Ran and importin a-dependent in the egg extract. We show that the addition of importin a to purified lamin B3 prevents the assembly of lamins in solution. Lamin assembly assays show that importin a prevents the self-association of lamins required to assemble lamin filaments into the typical paracrystals formed in vitro These results suggest a role for importin a in regulating lamin assembly and possibly modulating the interactions of lamins with lamin-binding proteins.
Note
Automated medline import. Electronic deposit: 2008-01-28.Electronic publication: 2008-01-30. Publication Date: 2008-03-28. Publication status: ppublish. Status: MEDLINE.
Automated medline import. Electronic deposit: 2008-01-28.Electronic publication: 2008-01-30. Publication Date: 2008-03-28. Publication status: ppublish. Status: MEDLINE.